Composite
Part:BBa_K5119073:Design
Designed by: Dhruv Aggarwal Group: iGEM24_Austin-utexas (2024-10-01)
pBTK1030 + AN-PEP + pelB Secretion plasmid
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 363
Illegal XbaI site found at 367
Illegal XbaI site found at 5273
Illegal XbaI site found at 6321
Illegal PstI site found at 6700 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 363
Illegal NheI site found at 981
Illegal NheI site found at 6491
Illegal NheI site found at 6514
Illegal PstI site found at 6700 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 363
Illegal BglII site found at 378
Illegal BamHI site found at 344
Illegal XhoI site found at 374 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 363
Illegal XbaI site found at 367
Illegal XbaI site found at 5273
Illegal XbaI site found at 6321
Illegal PstI site found at 6700 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 363
Illegal XbaI site found at 367
Illegal XbaI site found at 5273
Illegal XbaI site found at 6321
Illegal PstI site found at 6700
Illegal NgoMIV site found at 101
Illegal AgeI site found at 6776
Illegal AgeI site found at 6816 - 1000COMPATIBLE WITH RFC[1000]