Composite

Part:BBa_K5119073:Design

Designed by: Dhruv Aggarwal   Group: iGEM24_Austin-utexas   (2024-10-01)


pBTK1030 + AN-PEP + pelB Secretion plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 363
    Illegal XbaI site found at 367
    Illegal XbaI site found at 5273
    Illegal XbaI site found at 6321
    Illegal PstI site found at 6700
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 363
    Illegal NheI site found at 981
    Illegal NheI site found at 6491
    Illegal NheI site found at 6514
    Illegal PstI site found at 6700
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 363
    Illegal BglII site found at 378
    Illegal BamHI site found at 344
    Illegal XhoI site found at 374
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 363
    Illegal XbaI site found at 367
    Illegal XbaI site found at 5273
    Illegal XbaI site found at 6321
    Illegal PstI site found at 6700
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 363
    Illegal XbaI site found at 367
    Illegal XbaI site found at 5273
    Illegal XbaI site found at 6321
    Illegal PstI site found at 6700
    Illegal NgoMIV site found at 101
    Illegal AgeI site found at 6776
    Illegal AgeI site found at 6816
  • 1000
    COMPATIBLE WITH RFC[1000]